M28960_neisseria_meningitidis - SRA

SRX5019514: M28960_neisseria_meningitidis
1 ILLUMINA (Illumina MiSeq) run: 365,469 spots, 182.7M bases, 102.5Mb downloads

Design: Illumina sequencing libraries were prepared from extracted DNA by first shearing it to 600 bp using a Covaris LE220 focused ultrasonicator (Covaris, Inc., Woburn, MA). The sheared DNA was processed with the NEBNext ultra DNA library preparation kit following the manufacturers protocol (New England Biolabs, Ipswich, MA), using dual barcoding indices. Reads were trimmed using Cutadapt 1.8.1. (16) to remove adapter sequences and bases with a quality score of <Q20, and assembled with SPAdes v3.7 (17). Genomes were required to have an average coverage depth of 25x, and contigs were excluded as spurious if they had 10% of the genome-wide coverage depth.

Submitted by: Centers for Disease Control

Study: Collection of Neisseria meningitidis isolates

PRJNA498006 • SRP169098 • All experiments • All runs

show Abstracthide Abstract

A collection of Neisseria meningitidis isolates used for characterizing the capsule locus.

Sample:

SAMN09704721 • SRS4052375 • All experiments • All runs

Organism: Neisseria meningitidis

Library:

Name: M28960

Instrument: Illumina MiSeq

Strategy: WGS